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KMID : 0359320140540020113
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Korean Journal of Veterinary Research
2014 Volume.54 No. 2 p.113 ~ p.115
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Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces
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Kim Yong-Kwan
Kim Ha-Young
Jeon Byung-Yun
Lee Myoung-Heon
Bae You-Chan
Byun Jae-Won
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Abstract
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Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were 104, 105 and 102 CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.
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KEYWORD
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detection limit, invA, loop-mediated isothermal amplification assay, Salmonella
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